Electronic Supporting Information Artificial heme-enzyme with enhanced catalytic activity: evolution, functional screening and structural characterization

All Fmoc (9-fluorenylmethoxycarbonyl) protected amino acids, NovaSyn TG Sieber resin, and coupling reagents (HOBt, 1-Hydroxybenzotriazole, HATU, 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5b]pyridinium 3-oxid hexafluorophosphate, and PyBOP, (Benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate) were purchased from Novabiochem. All solvents, used in the synthesis and purification, were anhydrous and HPLC grade, respectively, and were supplied by Romil. Piperidine and scavengers (ethanedithiol, triisopropylsilane) were from Fluka. TFA (trifluoroacetic acid) and DIPEA (N, NDiisopropylethylamine) were from Applied Biosystems. Pre-coated silica G-60 plates, F254, used for thinlayer chromatography (TLC), were from Merck. Deuteroporphyrin IX was from Porphyrin Products. Iron (II) acetate was purchased from Sigma Aldrich. All buffer solutions were made by using water with a HPLC purity grade (Romil); phosphate salts (monobasic and dibasic) for buffers preparation, were provided by Fluka; TFE (2,2,2-Trifluoroethanol) was supplied from Romil. The substrates ABTS and H2O2 (30%, v/v), used in the catalytic assays, were purchased from Sigma and Fluka, respectively.